Molecular Kits Specific To The Beverage Industry
The use of PCR is now considered as the standard method for detecting nucleic acids from numerous microorganisms in a diversity of foods and beverages, both functional species as well as spoilers. Real time PCR is one of the most powerful, specific and reliable methods for the quantitative detection and identification of microorganisms at an early process stage to prevent spoilage and to maintain overall product quality.
At PIKA our goal is to make the best microbiology methods available to breweries of all sizes. All our PCR tests are low cost, outstandingly robust and simple to use, even in small breweries with no prior experience, helping you save time and money.
Our 4everyone Detection Kits can be run as individual samples so no reactions are wasted. Processing samples takes only 15 minutes, with results available within 75 minutes. All our PCR kit workflows and cycle parameters are standardized making them robust to follow and allowing the analysis of different analytes at the same time.
The 4 everyone Detection kit reagents, primers and probes come in a ready-made dried format in the PCR tubes for unrivalled ease of use and temperature stability. All PCR tests use the FAM channel for the detection of the target microorganisms and the VIC/HEX channel for an internal positive control. This allows the 4everyone Detection Kits to prevent false negatives due to ample inhibition, allowing you to be truly confident about negative results. Each kit contains 48 reactions but tests can be run individually.
In order to achieve the lowest detection limits, we recommend sample enrichment in our PCR certified FastOrange® enrichment media.
A typical workflow includes the following four steps;
To help understand how simple our DNA extraction for real time PCR testing is, please have a look at the video below:
We’ve listed our most popular selling PCR Detection Kits. Please click on any of the links below to be directed to product pages and for access to product manuals.
Top Selling Bacteria Detection Kits
We also offer a number of individual species PCR detection kits for bacteria, all of which are available to purchase in our shop. For further details of any of these please contact us.
- Alicyclobacillus acidoterrestris
- Lactobacillus brevis
- Lactobacillus coryniformis
- Lactobacillus perolens
- Pediococcus damnosus/inoponatus
- Lactobacillus acetotolerans
- Lactobacillus casei
- Lactobacillus lindneri
- Lactobacillus plantarum
- Lactobacillus backii
- Lactobacillus collinoides
- Lactobacillus parabuchneri (“frigidus”)
- Lactobacillus rossiae
Top Selling Yeast & Mold Detection Kits
Performance Functionality Kit
Instrumentation
Our Detection kits for real time PCR are intended for microtiter plate format in 100 µL and 200 µL tubes and can be used with real time PCR thermocycler instruments from almost all manufacturers. This means if you already have a real time PCR system in your laboratory, our stand-alone kits are most likely to be compatible for immediate use.
All PCR tests use the FAM channel (494/518 nm) for detection of the target microorganisms and the VIC/HEX channel (535/556 nm) for an internal control reaction. This allows 4everyone Detection Kits to prevent false negatives due to sample inhibition, allowing you to be truly confident about negative results.
The QuantStudio line from ThermoFisher is becoming an increasingly popular instrument offering simplicity and flexibility. PIKA have a close working relationship with ThermoFisher and we can work together to help get the instrumentation and workflow working in your laboratory with a turn-key solution giving you a single point of contact. For further details on the QuantStudio line please visit the ThermoFisher website www.thermofisher.com.
Many PIKA customers use the Open qPCR from Chai Biotechnologies, giving automated reading of results with no need to compare curves, lines or melting temperatures.
Given that Pall Corporation is discontinuing their GeneDisc® Rapid Microbiology System, customers are faced with some difficult decisions in order to continue their daily microbiological testing routines.
PIKA Weihenstephan believe we are uniquely positioned to help customers make the transition from Pall’s “closed” system to an “open” PCR testing environment with a high degree of confidence and minimal hassle. We might even save users time and money along the way.
Customers should be aware that, with the exception of the thermo cycler, all equipment necessary to prepare samples for the GeneDisc® system can still be used after switching to the PIKA Weihenstephan system.
Using PIKA 4everyone Detection Kits are very similar to those from GeneDisc® due to the fact that PIKA Weihenstephan collaborated closely with PALL in the evaluation of their GeneDisc® system. Both systems use similar primers and probes combinations, so users can confidently continue to use any Ct value limits they might have set for routine testing with GeneDisc®.
